Indian Society of Genetics & Plant Breeding
Randomly amplified polymorphic DNA markers were used
to assess the genetic diversity among 23 commercially
released varieties and breeding lines of garlic along with
onion as an out-group. A total of 100 operon decamer
primers were screened in duplicate and fourteen of these
primers were selected for further testing. The number of
bands per primer varied from 3 to 19, with an average of
11.5. Based on RAPD data, similarity values among garlic
genotypes ranged from 0.97 to 0.47 with a mean value of
0.72. A low degree of similarity (0.10-0.15) was observed
with onion. The UPGMA dendrogram constructed using
Jaccard’s similarity matrix discriminated all the genotypes
into four clusters. Cluster I comprised of 14 genotypes
with a similarity index of about 89%. Cluster II comprised
of only one line, SKAUG 151 that had a similarity index of
62% with Cluster I. Cluster III comprised of 8 garlic
genotypes at a distance of about 52%. Variation amongst
the garlic accessions was about 48%, whereas, with onion
it showed a dissimilarity of 90%. The clustering pattern of
garlic genotypes in the present study discriminated them
as per the vernalization requirement for bulb formation.
Keywords: Garlic, Allium sativum, genetic diversity, RAPD
Year: 2008
Volume: 68
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906
A. Khar, A. Asha Devi and K. E. Lawande info_circle
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