Characterization of novel soybean derived simple sequence repeat markers and their transferability in hyacinth bean [Lablab purpureus (L.) Sweet]

Published on Jul 20, 2018 01:22 AM

Simple Sequence Repeat (SSR) markers were developed
from soybean expressed sequence tag (EST) sequences
obtained from The European Molecular Biology Laboratory
(EMBL) database. Of the 1517 loci considered 764 (50.4%)
and 573 (37.8%) constituted tri and dinucleotide and, 73
(4.8%), 44 (2.9%), 38 (2.5%) and 25 (1.6%) represented
mono, hexa, tetra and pentanucleotide repeats,
respectively. Fifty of these SSR makers exhibited 100%
transferability in Lablab purpureus. Eight out of 50 loci
(16%) displayed length polymorphisms. BLAST results
showed that the function of only 15 EST sequences (30%)
of the selected 50 SSR sequences were known. The novel
EST-SSR will be useful for developing new molecular
markers of L. purpureus.

Info

Year: 2012
Volume: 72
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

L. M. Yao, L. D. Zhang, Y. L. Hu info_circle
Hu, B. Wang1 and T. L. Wu1 info_circle

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