Indian Society of Genetics & Plant Breeding
The genomic information available in barnyard millet is
very scarce though it is a rich source of highly digestible
proteins and dietary fibre with good amounts of soluble
and insoluble fractions. In the present investigation, 64
maize and finger millet genomic SSRs were used for cross
transferability analysis among barnyard millet cultivated
and wild species for identification of polymorphic markers,
syntenic regions, genetic diversity and population structure
analysis. Out of the 64 SSRs, only 39 (61%) were amplified
across the barnyard millet genotypes. The PIC values of all
the polymorphic loci for 24 barnyard millet genotypes varied
from 0.25 to 0.73 at an average of 0.35. Based on the values
of different parameters i.e., PIC values (>0.7), gene diversity
(>0.6), inbreeding coefficient (>0.27), the SSR loci bnlg2323
and bnlg2123 were observed to be highly polymorphic.
Polymorphism comparison of maize and finger millet SSRs
revealed that maize microsatellites were highly transferable,
more polymorphic and were able to distinguish barnyard
millet genotypes clearly. Results of population structure
and genetic diversity analysis were similar in differentiating
the barnyard millet genotypes into two groups. The
structure analysis showed that all genotypes were pure
lines (no admixture) while two (IEC514, and IEC409) had
mixture of alleles from other genotypes of population which
is depicted from the SSRs used in the study. The present
study enriched the barnyard millet genetic resources by
identifying suitable polymorphic markers of maize and
finger millet for diversity analysis, cultivar identification
and marker assisted breeding programmes.
Keywords: Cross transferability, barnyard millet, SSR markers, population structure, orthologs
Year: 2018
Volume: 78
Issue: 3
Article DOI: 10.31742/IJGPB.78.3.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906
B. Kalyana Babu, C. Rashmi and Salej Sood info_circle
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