Indian Society of Genetics & Plant Breeding
Studied the molecular divergence and develop DNA
fingerprints in selected popular fieldpea cultivars from
India. Those RAPD primers from the four sets (viz. OPP,
OPBA, OPAQ and OPH) which showed at least 75 percent
band polymorphism were selected for molecular diversity
analysis. Twenty four primers generated a total of 256
amplified fragments out of which 228 (89.06%) were
polymorphic. On an average, 10.67 bands were amplified
per primer. Cluster analysis based upon DNA amplification
polymorphism using Jaccard’s similarity coefficient and
UPGMA could unveil substantial amount of polymorphism
among the cultivars. Genotype specific bands were
represented in a diagrammatic form and can be used as a
reference fingerprint. The arithmetic mean heterozygosity
(Hav) value and marker index (MI) was found to be 0.592
and 6.317, respectively, indicating the efficiency and
usefulness of RAPD as a marker system.
Keywords: Fieldpea, molecular diversity, RAPD, genotype specific markers
Year: 2010
Volume: 70
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Hasan Tanveer info_circle
P. Ray Choudhury info_circle
G. P. Dixit info_circle
G. K. Jha info_circle
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