Indian Society of Genetics & Plant Breeding

Effect of Neovossia indica on embryo culture of wheat [Triticum aestivum (L.) em Theil] and its reflections on in vivo resistance

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In an effort to study the effect of Neovossia indica on
embryo culture of wheat [Triticum aestivum (L.) em Theil],
17, 20 and 23 days old embryos were excised from
immature grains of different wheat genotypes. These were
cuitured on modified MS medium in presence and absence
of the pathogen. The pathogen had no effect on callus
initiation and callusing frequency. Some of the 23 days
old embryos from the wheat genotypes formed a circular
inhibition zone (IZ) around themselves where no growth
of pathogen could be seen. The formation of IZ was
observed around every embryo excised from mature grains
and cultured in presence of the pathogen. The mean
diameter of IZ was found to be 3.19 cm in resistant, 2.26
cm in susceptible and 1.62 cm in highly susceptible
genotypes showing coefficient of infection less than 5%,
5 to 20 % and more than 20 %, respectively at adult
plant stage  

Keywords: Wheat, Neovossia indica, embryo culture, resistance.

Info

Year: 2006
Volume: 66
Issue: 2
 Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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