Indian Society of Genetics & Plant Breeding
A mutation breeding project was initiated with finger millet
varieties, VR 708 and GPU 26 using three doses each of
gamma rays (150, 300 and 450 Gy), ethyl methane
sUlphonate (0.15, 0.30 and 0.45%) and nitroso guanidine
(0.015, 0.030 and 0.045%) coupled with combination
treatments of 300 Gy gamma rays with 0.30% EMS or
0.030% NG. Fifteen selected M
2 plant progenies from each
of the eleven mutagenic treatments along with the parental
variety were evaluated in Ms generation. Four high yielding
M
s progenies from each treatment along with the parental
variety were evaluated for yield and eight component
traits in M
4 generation. In Ms generation, of the 165
progenies, 61 in VR 708 and 65 in GPU 26 produced
significantly higher yield than the parent and EMS
treatments produced more of such high yielding progenies.
In M
4, out of 44 progenies in each of VR 708 and GPU
26, 8 and 9 progenies showed superiority over the parental
variety in one or more traits, respectively. High frequency
of positive mutations was observed for 1000-grain weight,
finger length and fingers/ear in case of VR 708 and
fingers/ear and finger length in case of GPU 26. Moreover,
EMS treatments produced more superior mutants (28.93%
in VR 708 and 39.13% in GPU 26) in different traits than
the other mutagenic treatments. Among the mutagenic
treatments, the frequency of high yielding progenies in
M
s and M4 generations were higher in 0.30% and 0.45%
EMS, 0.030% NG and combination treatment of 300 Gy
gamma rays + 0.30% EMS
Keywords: Finger millet, induced mutations, superior micromutants, mutagenic treatments
Year: 2007
Volume: 67
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
K. C. Muduli and R. C. Misra info_circle
Download PDF