Indian Society of Genetics & Plant Breeding

Efficiency of molecular markers in identifying fertility restoration trait of WA-CMS system in rice

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To assess the efficiency of different molecular markers
which are linked to fertility restorer genes Rf3 and Rf4 of
WA-CMS system, 103 breeding lines with no information
on fertility restoration were screened with the help of
molecular markers linked to major fertility genes Rf3 and
Rf4. The breeding lines were crossed with APMS6A and the
F1s were evaluated for pollen and spikelet fertility to identify
restorers and maintainers.The SSR primer RM6100 linked
to Rf4 gene of chromosome 10 and RM10313 linked to Rf3
gene of chromosome 1 showed eighty five and eight one
percentage efficiency respectively in identifying restorer
lines. Therefore,these markers are useful tool for evaluating
large number of breeding lines to know about their fertility
restoration in a short period of time without generating
and evaluating large number of test crosses.The potential
restorers may be identified with hundred percentage
efficiency based on molecular screening itself, if candidate
genes based markers are developed and validated for both
Rf4 and Rf3 genes

Keywords: Fertility restoration, Rf4, Rf3, molecular markers, WA-CMS

Info

Year: 2013
Volume: 73
Issue: 1
 Article DOI: 10.5958/j.0019-5200.73.1.012
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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