Indian Society of Genetics & Plant Breeding
In the present investigation genetic diversity amongst 190
pearl millet accessions viz., 95 maintainer (B line) and 95
restorer lines (R line) was analyzed using SSR markers. A
total of 40 SSR primers were used for screening, out of
which 34 were polymorphic. PIC value ranged from 0.002
(PSMP 2068) to 0.749 (CTM 60) with an average of 0.445.
AMOVA showed greater variance represented by individuals
within the group (65%) and between group (B and R lines)
variance was <35%. The neighbor joining tree based on
dissimilarity matrix of germplasm lines differentiated B from
R lines into two major clusters with few exceptions of R
lines grouping with B lines and vice versa. Genetic
dissimilarity estimates ranged from 0.12 (RPR 22; IPC 917)
to 0.85 (ICMR 10222; ICMB 97333). The maintainers ICMB
97333, RPB 6 and ICMB 92777 and restorers ICMR 10222,
RPR 25, RPR 56, RPR 62 and RPR 40 were identified as most
distinct and divergent which could be used as parents for
heterosis breeding.
Keywords: Genetic diversity, maintainers, restorer, pearl millet, AMOVA
Year: 2018
Volume: 78
Issue: 3
Article DOI: 10.31742/IJGPB.78.3.16
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Ruchika Bhardwaj, Tosh Garg, Ejaz A. Malik, Yogesh Vikal, R. S. Sohu and S. K. Gupta info_circle
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