Inter-specific crosses, viz., Jayadhar (2x) x BCS-23 (4x),
A82-1-1 (2x) X BCS-23 (4x), Jayadhar (2x) x G. australe
(2x), Jayadhar (2x) x G. gossypoides (2x) and Abadhita
(4x) x G. australe (2x) were attempted. Plant growth
hormones like Indole acetic acid, Naphthalene acetic acid
and Gibberellic acid with three concentrations were applied
to crossed buds to prevent shedding, Very low frequency
(0.047-0.15 %) of in vivo cross recovery between cultivated
diploid and tetraploid and cultivated and wild species,
respectively, indicated the presence of post-zygotic
incompatibility. Application of PGRs particularly GA3 @
0.1 % in 2x x 4x crosses prevented abscission of crossed
buds at the highest frequency. There was 36.69 % boll
retention as against only 2.70 in control. Phenotyping
revealed the intermediate nature of hybrids for most of
the morphological characters. Presence of multiple nuclei
at tetrad stage (pentad and hexad) was observed
cytologically due to abnormal segregation of laggards.
Keywords: Colton, inter-specific hybridization, incompatibility, plant growth hormones, phenotyping, multiple nuclei.
Year: 2005
Volume: 65
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
M. K. Rajashekhar, I. S. Katageri and B. M. Khadi info_circle