Marker assisted gene pyramiding of leaf rust resistance genes Lr24, Lr28 along with stripe rust resistance gene Yr15 in wheat (Triticum aestivum L.)

Published on Aug 10, 2018 12:31 PM

Two highly effective genes for leaf rust resistance viz.,
Lr24, Lr28 and a stripe rust resistance gene Yr15 were
selected for pyramiding in the background of a susceptible
but high yielding bread wheat variety HD2877. The
screening against most virulent pathotypes of leaf rust
77-5 (121R63-1) and stripe rust, 46S119 and 78S84
indicated that all the three genes confer a high degree of
seedling and adult plant resistance. The use of molecular
markers, namely, SCS1302607, SCS421570 and Xgwm273
validated the presence of resistance genes, Lr24 and Yr15
in Sunstar*6/C80-1//V763-2312 and Lr28 in HW2033 both
being donors. The application of molecular markers
facilitated identification of individual plants in three-way
cross (HD2877 x Sunstar*6/C80-1//V763-2312) x HW2033,
BC1 -F1 and BC2 -F1 generations possessing the targeted
genes. Finally eight plants were selected in BC2 -F2
generation carrying the desired resistance genes, Lr24,
Lr28 and Yr15 in different combinations in the background
of HD2877. The availability of combination of major rust
resistance genes in desirable background would facilitate
the strategic deployment of wheat varieties to achieve
durable resistance.
 

Keywords: Triticum aestivum L., pyramiding, leaf rust, stripe rust, resistance genes, molecular markers

Info

Year: 2010
Volume: 70
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

P. Revathi info_circle
S. M. S. Tomar , Vinod info_circle
N. K. Singh info_circle

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