Indian Society of Genetics & Plant Breeding

Molecular diversity in Carthamus species and species identification using RAPD markers

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The molecular genetic relationship between four
Carthamus species and the cultivated variety A-1 was
determined using 44 RAPD primers.The RAPD primers
produced a large number of markers. On an average
11.65 bands per primer were produced. Totally 83.75
percent of the markers were polymorphic. The
polymorphic markers were also used to assess the
inter-relationships of the species and to estimate the
overall genetic variation in the species.The
Carthamus
oxyacantha
and. Carthamus palaestinus were more
closely related to the cultivated species supporting the
previous taxonomic studies based on cytogenetics.
These two species were reported to be the proginators
of cultivated
Carthamus tinctorius L. The Carthamus
glaucus
L., which has a chromosome number of n =
10; as against n =12 of the cultivated species, formed
a distinct solitary cluster and showed least similarity
with cultivated species and its progenitors. The
Carthamus species (unidentified) was distinct from all
the other
Carthamus species studied and formed a
solitary cluster. The study revealed the significant
genetic differences in the genome of
Carthamus
species and species specific markers were identified.
The cultivated varieties A-1 and A-2 were crossed to
different
Carthamus species and interspecific hybrids
were produced. The species specific markers were
useful in identifying true interspecific hybrid plants
  

Keywords: Carthamus species, genetic diversity, RAPD marker, interspecific hybridization, safflower

Info

Year: 2007
Volume: 67
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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