Markers linked to QTLs are useful in practical plant breeding,
only if they get validated in genotypes of independent
populations and diverse genetic backgrounds. Forty one
SSR markers reported linked to QTLs for grain iron (Fe)
and zinc (Zn) concentration in wheat were analysed. Only
16 of them showed polymorphism and the remaining 25
turned out to be monomorphic in 48 wheat genotypes used
in the present study. Single marker analysis (SMA) for the
16 polymorphic markers was carried out to assess the
linkage between marker and the trait, based on which two
markers (Xbarc186 and Xbarc74) for grain Fe concentration
and three markers (Xgwm3, Xwms149 and Xgwm538) for
grain Zn concentration were validated in the present study.
The phenotypic variations explained by Xbarc186, Xbarc74,
Xgwm3, Xwms149 and Xgwm538 were 40.2% and 19.8%,
10.7%, 21.7%, and 39.6%, respectively. The validation of
these SSRs may be useful in breeding wheat with high
grain Fe and Zn concentration.
Keywords: Validation, QTL, iron, zinc, SSRs
Year: 2018
Volume: 78
Issue: 3
Article DOI: 10.31742/IJGPB.78.3.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Gopalareddy Krishnappa, Anju Mahendru Singh, A. K. Ahlawat, S. K. Singh, Poornima Sharma, R. B. Shukla and G. P. Singh info_circle