Phenotyping and microsatellite marker analysis of CSR10 (salt tolerant indica) × HBC19 (Taraori Basmati) F6 lines obtained using single seed descent method

Abstract

A population in F6 generation comprising of 201 lines derived from the cross between CSR10 (salt tolerant, indica) and Taraori Basmati (HBC19) using single seed decent method, was evaluated for variation in agronomic and Basmati grain quality traits and for microsatellite allelic profile in relation to the parental rice varieties. Substantial variability was observed for plant height, productive tillers per plant, 1000-grain weight, yield per plant, kernel length (L) and breadth (B) and L/B ratio. The grain yield per plant showed positive correlation with number of productive tillers per plant and 1000-grain weight. Kernel length and breadth respectively had positive and negative correlations with L/B ratio. The path coefficient analysis recorded that number of productive tillers per plant, plant height and 1000-grain weight were the main contributors towards grain yield. A total of 20 randomly selected F6 plants were subjected to SSR marker analysis using 15 SSR markers covering all the chromosomes. The F6 plants had an allele from either of the two parental lines (homozygous condition) or alleles from both the parental rice varieties (heterozygous condition). At three SSR loci new/recombinant alleles were observed, which indicate the active recombination between genomes of two rice varieties. SSR allelic profile based two dimensional principal component analyses demonstrated high level of diversity between CSR10 and HBC19 with the 20 CSR10 × HBC19 F6 plants interspersed between them. SSR analysis also showed an average homozygosity of 93.3% in F6 lines, which is close to expected value of 98%.