Development and molecular marker analysis of Karnal bunt resistant near isogenic lines in bread wheat variety PBW 343

Abstract

Karnal bunt (KB) resistant near isogenic lines (NILs) were developed using the stock (KBRL 22) with high KB resistance and the widely grown wheat cultivar (PBW 343) as recipient. Genetic analysis in BC5F2 and BC5F3 populations revealed involvement of up to three additive genes for KB resistance. Single plants from lines which were uniform for resistance and susceptibility in BC5F3 , were advanced to BC5F4 and screened further for establishment of extreme NILs. The KB resistant NILs were screened for presence of donor alleles at 93 polymorphic SSR loci using the recipient parent (PBW 343) as control. Donor alleles of four markers; Xgwm99 (1AL), Xgwm149 (4BL), Xgwm174 (5DL) and Xgwm340 (3BL) showed their presence in the resistant pool. These four markers were run on the 11 resistant and 10 susceptible BC5F4 progenies. Resistant and susceptible phenotypes showed association with three of the four markers viz., Xgwm 99, Xgwm 174 and Xgwm 340 as indicated by the Chi square contingency test.