Transformation of genetical ratios in crosses involving X-ray irradiated pollen grains in rice (Oryza sativa L.)

Abstract

Early reports on radiation dependant-genetical transformation indicated that sublethal levels of irradiated pollen grains allow development of normal hybrid progeny but in sUbsequent generations they occasionally express paternal characters due to differential inheritance of donor genes. However, detailed genetic analysis has not yet been followed up for confirmatory analysis of genetic transformation especially in rice (Oryza sativa L). Presently different crosses of (normal and treated) Cherumodan (green) x Japan Violet (purple) were effected by using X-ray irradiated pollen grains of the male parent in the treated crosses. The crosses were studied up to F3 of the control and M3F3 of the treated generations for inheritance/differential inheritance for fourteen morphological characters. The results showed transformation of genetical ratios in respect of ten morphological characters in the M2F2 populations when compared to the control. Observations recorded In the FiM2F2 are duly confirmed by the results obtained from the FiM3F3 generations. It could be concluded that irradiation might have caused allelic differences as to transform genetical ratios concerned as discussed.