Protein electrophoregrams use in soybean [Glycine max (L.) Merrill] cultivar identification

Abstract

Cultivar identification with respect to its genetic purity is important in national and international seed and breeding programmes. Different cultivars are most commonly identified on the basis of taxonomic differences of seed, seedling and mature plants. These parameters are highly susceptible to environmental, physiological and ecological conditions. Therefore, precise characterization of varieties should be done at both levels, viz. morphological as well as biochemical levels [1]. Proteins being the direct gene products reflect the genomic composition of varieties accurately to some extent and therefore, are ideal for genotypic distinctness. Biochemical approach like gel electrophoresis of proteins is a powerful tool to distinguish varieties. In the present investigation genetic diversity among soybean [Glycine max (L.) Merrill] varieties was determined on the basis of their electrophoregrams through Discontinuous Polyacrylamide Slab Gel Electrophoresis (DPAGE) method [2].