Radiation induced pod and seed mutants and MMS induced closed flower mutant in broad bean (Vicia faba L.)

Abstract

Present study reports induced pod and seed mutants obtained at 10kR of gamma rays and a closed flower mutant at 0.04% of MMS in Vicia faba L. variety minor. Dry (10-12% moisture) and healthy seeds of Vicia faba L. var. minor were irradiated with four different doses (10, 20,30 and 40kR) of gamma rays. Another set of seeds presoaked in distilled water for 12 hours was treated with four different concentrations (0.01, 0.02, 0.03 and 0.04%) of MMS for 6 hours. One set of seeds was kept untreated for comparison. All the sets of seeds were sown following complete randomized block design (CRBD) to raise the M1 generation in rabi season of 2003-2004. The seeds were collected individually from the M1 plants and sown in field to raise M2 and from individual M2 plants M3 generation in the same season of 2004-2005 and 2005-2006.