Efficacy of Benzotriazole as a chemical hybridizing agent in chilli (Capsicum annuum L.), cotton (Gossypium arboreum L.) and radish (Raphanus sativus L.)

Abstract

A group of 48 wheat varieties have been used in the present study to assess the morphological and molecular diversity. These wheat genotypes belonging to durum and aestivum species were grown in RBO with two replications in three rows of 2m length with spacing of 23 cm between rows. Observations on five randomly selected plants were recorded in each entry for quantitative traits viz., days to 50 per cent flowering, days to maturity, plant height, peduncle length, flag leaf length, flag leaf width, number of productive tillers per meter, spike length, awn length, number of spikelets per spike, number of grains per spike, 1000-grain weight and grain yield per plot. Mahalanobis 0 2 statistic was used for assessing the genetic divergence between genotypes [1]. For assessing the molecular diversity, the ONA was extracted from 10-12 days old wheat seedlings following CTAB extraction method [2]. Thirty random decamer primers viz., OPA 01 to 12, OPP 01 to 10, OPK 03 and 07 and OPF 01, 02, 03, 05, 10, 15 were used to screen the wheat genotypes. As many as 11 primers exhibited polymorphism and 2 primers showed monomorphism, while the remaining primers did not produce detectable bands. RAPO profiles were visually scored for the presence or absence of bands across the lanes. The similarity coefficients were used for cluster analysis to depict the relationship among the genotypes using unweighed pair group method for arithmetic average (UPGMA). All the computational and statistical analyses were performed using NTSYS programme.