Effect of growth regulators on androgenesis and regeneration in rice (Oryza sativa L.)

Abstract

Seven selected hybrids viz., CR 1009/HA 891037, CR 1009ITNAU 841434, ADT 40/AS95035, CO 43/ADT 43, Improved White Ponni/CB 97033, Improved White Ponni IIET 15341 and Improved White Ponni ICB(DH) 95298 were anther cultured for the production of doubled haploid lines. Maximum callus induction was observed in Improved White Ponni ICB(DH) 95298 (32.67 per cent) and minimum in CR 10091 HA 891037 (10.77 per cent). The cytokinin treatment with 2.0mg/l BAP and 2.0mg/l GA3 accounted for maximum regeneration. The frequency of callus induction was distinctly influenced by the genotype and growth regulators. Callus induction occurred 20 to 25 days after inoculation and maximum number of calli per tube were formed between 30 to 75 days. The calli that emerged in 50 days had good competitive and morphogenetic potential for green plantlet regeneration and those calli emerging after 75 days had poor regeneration ability. Among the seven hybrids cultured, green plant regeneration was observed in only three hybrid combinations.