Genetic diversity analysis in Indian diploid cotton (Gossypium spp.) using RAPD markers

Abstract

Random amplified polymorphic ONA (RAPO) analysis was employed in commercially released 18 cultivars of diploid cotton belonging to two species i.e. Gossypium arboreum and Gossypium herbaceum. The 20 primers, selected from 60 initially screened, generated 224 amplification products of which 127 amplicons were polymorphic. All primers produced polymorphic amplification prOducts, however, the extent of polymorphism varied with each primer. Statistical analysis was carried out using NTSYS-pc software and a dendrogram was generated using Jaccard's similarity coefficients. The values for similarity coefficients ranged from 0.50 to 0.99. Cluster analysis showed clear-cut separation of the genotypes of the two species. G. herbaceum cultivar Jayadhar did not cluster with any other cultivar. G. arboreum cultivars formed three clusters (II, III and IV). Cluster II contained three out of four cultivars from the same place, whereas cluster IV contained cultivars from Punjab and Haryana.