Detection of a protein marker for screening of MYMV resistant mungbean genotypes

Abstract

Mungbean Yellow Mosaic Virus (MYMV) is the most destructive disease in mungbean, Vigna radiata (L.) Wilczek, in the Indian subcontinent and adjacent countries of South East Asia [1]. It may cause yield losses even to the tune of 100%. Use of resistant genotypes is the most effective alternative to mitigate this yield loss. Legume seeds contain 70% globulin, 15-20% albumin and 15-20% glutelin [2]. Association of seed proteins with host response to biotic stress in crop plants has already been reported [3, 4, 5]. Analysis of seed proteins using sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SOS-PAGE) in mungbean has been employed for depiction of the seed protein profile [6] and characterization and identification of varieties [6, 7] and elucidation of evolution and phylogentic relationship [6, 8]. This approach could also be used for screening of MYMV resistant genotypes in this crop.