Effect of media composition on In vitro multiplication of sugarcane varieties

Abstract

Healthy and contamination free in vitro sugarcane plantlets of varieties Co 86032 and CoN 91132 were used in order to study the effect of different levels of Kinetin, Benzine Adenine (BA) and Coconut water on shoot multiplication, length of main shoot and number of leaves on main shoot. The most widely accepted MS medium [1] was supplemented with three levels of kinetin i.e. 0.5, 1.0 and 1.5 mg/lit; four levels of BA i.e. 0.5, 1.0, 1.5 and 2.0 mg/I and two levels of coconut water i.e. 10 and 20 per cent, thus making 25 treatment combinations (24 + control) arranged in three sets (Table 1). The MS basal medium devoid of kinetin, BA and coconut water was used as control.