Screening of wheat varieties for use as donors for production of near isogenic lines with different seed protein alleles

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Published: Aug 25, 2003
DOI: https://doi.org/.
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Keywords:
Wheat, seed storage proteins, allelic variation, SDS-PAGE. NILs

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M. Rathore
Department of Molecular Biology and Genetic Engineering, GBPUAandT, Pantnagar 263 145
D. P. Mishra
Department of Molecular Biology and Genetic Engineering, GBPUAandT, Pantnagar 263 145
N. K. Singh
Department of Molecular Biology and Genetic Engineering, GBPUAandT, Pantnagar 263 145

Abstract

Sequential extraction of water-soluble albumins, salt-soluble globulins (triticin), alcohol-soluble gliadins and acid-soluble glutenins, the four major classes of wheat seed storage proteins, was done from 31 wheat cultivars. SOS-PAGE of the protein fractions showed high variation in the banding pattern. Varieties with different alleles in comparison to the recurrent parent HO 2329 were chosen for all the four fractions of seed storage proteins. A total of 12 cultivars were finally chosen to make NILs for forty different seed storage protein alleles in HO 2329.

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How to Cite
Rathore, M., Mishra, D. P., & Singh, N. K. (2003). Screening of wheat varieties for use as donors for production of near isogenic lines with different seed protein alleles. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 63(03), 206–208. https://doi.org/.
Issue
Vol. 63 No. 03 (2003): Indian Journal of Genetics and Plant Breeding
Section
Research Article
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