CHROMOSOME ANALYSIS OF CULTURED CELLS OF VIGNA MUNGO (L). HEPPER

Abstract

A study on the variation in chromosome number of the cells from callus tissues of Vigna mungo L. Hepper for cotyledon, hypocotyl and embryonal axis explant was undertaken. The variation induced by treatment 2, 4-D and kinetin treatments at 10, 20 and 30 days was studied in all the three explants. The chromosome number showed a wide range of variation, however, the frequency of diploid cells (2n = 22) was higher in all the cultures obtained from each type of explant. Frequency of tetraploid cells was higher as compared to aneuploid and cells with higher ploidy level, the tetraploid cells increased with the increase in age of the callus in all the explants. The frequency of aneuploid and higher polyploid cells also showed increase with increase in age of culture. Plant regeneration was achieved after 30 days of inoculation. Hypocotyl explant cultured on MS medium supplemented with 0.5 mg/l kinetin identified as the best treatment for the induction of somaclonal variation in Urd been.