STUDY ON EFFECT OF GENOTYPE AND CULTURE MEDIUM ON CALLUS FORMATION AND PLANT REGENERATION IN RICE (ORYZA SATIVA L.)

Abstract

A study on the effect of genotype and culture medium on callus induced in seed explants of ten rice cultivars indicated significant variances due to genotypes, media and genotype x medium interactions (except callus induction frequency) for eight characters related to callusing and plant regeneration. Among the genotypes, Pusa Basmati 1 gave the best overall callusing response. For plantlet regeneration, variety Pant Dhan 4 recorded the highest number of green spots and shoots while Sarju 52 had the highest potential for the length of longest shoot and rooting. Likewise the MS medium supplemented with 2.0 mgl- t , 2,4-0 for callusing and 2.0 mgl- t IAA plus 3.0 mgl- t KN (MS (3)) for shooting while 3.0 mgl- t IAA plus 4.0 mgr1 KN (MS (4)) for rooting gave the best results. Some of the selected combinations like Pant Ohan 4 on MS medium supplemented with 2.0 mgl- t 2,4-0 plus 1.0 mgrt KN displayed excellent callusing capacity while Pant Ohan 4 on MS (3) and MS (4), Sarju 52 on MS (4) and Pusa Basmati 14.0mgrt lAA plus 4.0 mgl- t KN (MS (5)) exhibited best regeneration capacity, and therefore, offer good scope for high totipotency using appropriate genotype and media with proper combination of hormones.