RFLP FINGERPRINTING OF ORYZA AUSTRALIENSIS GENOTYPES USING CULTIVATED RICE GENOMIC PROBES

Abstract

Four accessions of O. australiensis with EE genome were examined for restriction fragment length polymorphism (RFLPs). Fifteen RFLP clones of O. sativa, with known chromosomal positions showed RFLPs among the accessions. However, 54.60/. monomorphic banding patterns indicated conserved nature of DNA of O. australiensis genome. The differences in electrophoretic banding patterns were parsimoniously interpreted as the result of 108 mutations. Three RFLP clones (RGll8, RG190, RG207) revealed a number of RFLPs among the accessions which could fingerprint the four genotypes. The genetic relationship between the accessions were determined by the positions of the accessions in the phylogenetic tree and by computation of mutation distances between the accessions. We found that the accession 100882 is closer to the accession 101397 in terms ofgenetic distance than the accessions 103318 and 101410.