Genetic divergence studies in mungbean (Vigna radiata L. Wilczek) using morpho-physio and molecular markers to identify drought tolerant genotypes

Abstract

Sixty mungbean genotypes were evaluated for genetic divergence under limited moisture condition during summer season. Based on 19 phenotypic markers, hierarchical cluster analysis identified nine clusters containing one to 15 genotypes. Principal factor analysis reduced the nineteen variables into six principal components explaining 74.66% of the total variability. Seed yield per plot, harvest index and number of seeds per pod loaded highly on the PC 1; photochemical efficiency and canopy temperature depression on PC 4 and membrane stability index, total chlorophyll content and 100 seed weight on PC 5. Genotypes MH 810, MH 721, MH 736, M 395, SML 668, Pusa 9972 were found promising with regard to yield and drought tolerance traits. Out of 31 SSR primers used, only 18 were polymorphic and amplified 74 alleles. Primer CEDG067 exhibited maximum PIC value (0.84). The NTSYS-pc UPGMA cluster analysis divided the sixty genotypes into nine distinct groups.