An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen
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Abstract
There is no robust and quick technique available for testing germination of wheat pollen. In this study, Brewbaker and Kwack (BK) pollen germination medium (PGM) was adjusted with a-amino caproic acid (EACA), peptone water and maltose to develop an improved medium for wheat. Germination of pollen in this PGM ranged from 92% to 98.2% depending upon genotype pollen germination media (PGM). This is the first report of PGM which has >95% wheat pollen germination in vitro.
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Jayaprakash, P., Annapoorani, S., Vikas, V. K., Sivasamy, M., Kumar, J., Saravannan, K., Punniakotti, E., & Sheeba, D. (2015). An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 75(04), 446–452. https://doi.org/10.5958/0975-6906.2015.00072.3
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Research Article
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