In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations
Main Article Content
Abstract
Bunium persicum (Boiss.) B. Fedtsch. is one of economically important member of the Apiaceae family with a tuberous root. Production of this plant is restricted due to seed dormancy and long juvenile time. The objective of this study was to determine the best media and optimum concentration of sucrose for in vitro microtuberization of Black Zira. Therefore, different basal media (Murashige and Skoog (MS), Woody Plant Medium (WPM), Gamborg’s B5, and Gupta and Durzan’s DCR) with 3% sucrose were used for microtuber induction. Then best media was tested by varying concentrations of sucrose (3, 6, 9, and 12%) to increase morphological characteristics diameter and weight of microtubers. The experiment was done in a Randomized Complete Block Design with four treatments and 8 replications per treatment. According to the results obtained in this study, the highest mean of the root length (4.5196 cm), tuber length (1.175 cm), tuber width (0.3575 cm), tuber diameter (4.1 mm), and tuber weight (0.1472 g) after six weeks was observed in the WPM medium. Among the four concentrations of sucrose in WPM medium supplemented with 6% sucrose indicated a better response than the other concentrations in terms of microtuber diameter (7.675 mm), and weight (0.2687 g) average and was found optimum. Therefore, the WPM was recognized as the best medium for micro-tuberization.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Abbasian. A., Ahmadi. A., Abbasi. A.R. and Darvishi. B. 2018. Effect of various phosphorus and calcium concentrations on potato seed tuber production. Journal of Plant Nutrition. 41:1765-1777.
Ali. S., Khan.N., Nouroz.F., Erum.S. and Nasim. W. 2018. Effects of sucrose and growth regulators on the microtuberization of cip potato (Solanum tuberosum) germplasm. Pak. J. Bot, 1:763-768.
Ab. Rahman, Z., Ali. MSM., Ghazalli. MN., Awang. K. and Othman. AN. 2018. Optimization of Culture Media Formulations for Micropropagation of Lepisanthes fruticosa. Biosci Biotechnol Res Asia. 11: 51-58. http://dx.doi.org/10.13005/bbra/2607
Aseesh. P. and Tamta. S. 2013. Effect of pre-sowing treatments on seed germination in Quercus serrata Thunb and Quercus semecarpifolia Sm. Biodivers. Conserv. Biodivers Conserv. 5:791-795. https://doi.org/10.5897/IJBC2013.0605.
Aslam. A., Ali. A., Naveed. NH., Saleem. A. and Iqbal. J. 2011. Effect of interaction of 6-benzyl aminopurine (BA) and sucrose for efficient microtuberization of two elite potato (Solanum tuberosum L.) cultivars, Desiree and Cardinal. Afr J Biotechnol. 10:12738-12744. https://doi.org/10.5897/AJB11.2005.
Assareh. MH. and Sardabi. H. 2005. Macropropagation and micropropagation of Ziziphus spina-christi. Pesqui. Agropecu. Bras. 40: 459-465. https://doi.org/10.1590/S0100-204X2005000500006.
Bagheri. A., Moshiri. F. and Khosravinia. S. 2014. Invistigation on reaction of explants and plant growth regulators on callus induction, rooting and in vitro regeneration of Bunium persicum (Boiss.) B. Fedtsch. Plant Biotechnol J 3:53-61.
Banyal. S. and Sharma. S. .2020. Assessment of the Efficacy of Some Physico-Chemical and Hormonal Treatments for Dormancy Removal and Germination Improvement in Bunium Persicum (Kala Zeera). Intl. J. Chem. Stud, 8:2330-2337.
Bansal. S. Sharma, K. Gautam. V., Lone. AA., Malhotra. EV., Kumar. S. and Singh. R . 2021. A Comprehensive Review of Bunium persicum: A Valuable Medicinal Spice. Food Rev. Int 1-20. https://doi.org/10.1080/87559129.2021.1929305
Davara Monali. A., Polara. K., Ribadiya. TR., Vadaliya. BM. and Vekariya. L. 2019. Effect of potassium and zinc on growth, yield and quality of coriander (Coriandrum sativum L.). Int J Chem Stud. 7:292-295
Desai. P., Patil. G., Dholiya. B., Desai. S., Patel. F. and Narayanan. S. 2018. Development of an efficient micropropagation protocol through axillary shoot proliferation for pomegranate variety ‘Bhagwa’. Ann Agrar Sci. 16: 444-450. https://doi.org/10.1016/j.aasci.2018.06.002
Emamipoor. Y. and Maziah. M. 2014. An efficient method in breaking of dormancy from B unium persicum (Boiss) Fedtsch seeds: a valuable herb of Middle East and Central Asia. Asian Pac J Trop Biomed. 4:642-649. https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0042
EL-Morsy. A.H.A., El-Metwaly. H.M.B. and El-Said. E.M. 2020. Effect of Foliar Spray with Calcium and some Antioxidants on Growth, Yield and Yield Quality of Potato. Journal of Plant Production, 11: 797-803.
Fufa. M. and Diro. M. 2013. The effects of sucrose on in vitro tuberization of potato cultivars. Adv Crop Sci Tech. 1:2. http://dx.doi.org/10.4172/2329-8863.1000114
Gamborg, OL. and Eveleigh. D. 1968. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can J Biochem. 46:417-421 https://doi.org/10.1139/o68-063
Gani. R., Bhat. ZA., Dar. MA. and Dar. MA. 2020. Pharmacognostic and Phytochemical Characteristics of The Fruits of Bunium persicum (Boiss.) B. Fedtsch, Growing Wild in Kashmir Valley, India. Int J Pharm Investig. 10:13-16. https://doi.org/10.5530/ijpi.2020.1.3
Geoge. EF. and Sherrington. PD. 1984. Plant propagation by tissue culture: handbook and directory of commercial laboratories. Exegetics Limited.
Gupta. PK. and Durzan. DJ. 1985. Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep. 4:177-179.
Haider. MU., Farooq. M., Nawaz. A. and Hussain. M. 2018. Foliage applied zinc ensures better growth, yield and grain biofortification of mungbean. Int J Agric Biol. 20:2817-2822.
Hirschi. KD. 2004. The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiol. 136: 2438-2442. https://doi.org/10.1104/pp.104.046490.
Husain. S., Shah. S.A.H., Asghar. S., Hussain. N., Ali. N., Hussain. I., Rafiq. S., Shah. S., Imtiaz. M., Ali. M. and Jala. F. 2017. Micro-Tuberization of Four Potato (Solanum tuberosum L.) Cultivars through Tissue Culture. Research & Reviews. Journal of Botanical Sciences, 6:pp.35-40.
Imani. A., Qhrmanzadeh. R., Azimi. J.and Janpoor. J. 2010. The effect of various concentrations of 6-benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction. AEJAES. 8:457-459.
Kang. Y., Lee. K., Choi. J., Komakech. R., Min. J., Ju. S., Kim. SW., Youn. C., Kim. Y-G. and Moon. B. 2018. Maximizing seedling and root tuber production in Polygonum multiflorum for use in ethnomedicine. S Afr J. 119:119-131. https://doi.org/10.1016/j.sajb.2018.08.016.
Mardani. H., Ziaratnia. SM., Azizi. M., Aung. HP., Appiah. KS. and Fujii. Y. 2015. In vitro microtuberization of Black Zira (Bunium persicum Boiss.). AJB. 14:2080-2087. https://doi.org/10.5897/AJB2015.14427.
Makau. F.M., Mwangi. M., Oyoo. M.E., Kibe. A.M. and Oggema. J. 2022. Effects of Sucrose and Gibberellic Acid on Growth and Survival of Local Potato (Solanum tuberosum L.) Varieties in vitro in Kenya. European Journal of Biology and Biotechnology, 3:.13-18.
McCown. B. 1981. Woody Plant Medium (WPM)-a mineral nutrient formulation for microculture for woody plant species. Hort. Sci. 16: 453.
Murashige. T. and Skoog. F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nazir. R., Gupta. S., Dey. A., Kumar. V., Gupta. AP., Shekhawat. MS., Goyal. P. and Pandey. DK. 2022. In vitro tuberization, genetic, and phytochemical fidelity assessment of Dioscorea deltoidea. Ind Crops Prod. 175:114174. https://doi.org/10.1016/j.indcrop.2021.114174.
Phillips. G.C. and Garda. M. 2019. Plant tissue culture media and practices: an overview. In Vitro Cell.Dev.Biol.-Plant. 55: 242–257. https://doi.org/10.1007/s11627-019-09983-5.
Purohit. S., Joshi. K., Rawat. V., Bhatt. ID. and Nandi. SK. 2020. Efficient plant regeneration through callus in Zanthoxylum armatum DC: an endangered medicinal plant of the Indian Himalayan region. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology. 154:288-294. https://doi.org/10.1080/11263504.2019.1610107.
Purwito. A., Efendi. D., Ermayanti. T. 2021. Growth response of four accessions of Moringa oleifera Linn shoots cultured on various basic media. IOP Conf Ser Earth Environ Sci. 741:012054. https://doi.org/10.1088/1755-1315/741/1/012054.
Rehman. HU., Gill. MIS., Dhillon. WS. and Bedi. S. 2014. Micropropagation of Pathernakh (Pyrus pyrifolia (Burm F.) Nakai) pear using explants obtained from forced cuttings. Int J Agric. Sci 2:54-65.
Rezaee. A. and Kamali. K. 2014. A new commercial protocol for micropropagation of myrtus tree. ABR. 5:73-79.
Saeidnejad. A-H., Khajeh-Hosseini. M. and Askarzadeh. M-A. 2013. Breaking dormancy of seeds from eight populations of Bunium persicum (Apiaceae). Seed Sci Technol. 41: 452-457. https://doi.org/10.15258/sst.2013.41.3.11
Sharma. R. and Sharma. S. 2010. Effect of storage and cold-stratification on seed physiological aspects of Bunium persicum: A threatened medicinal herb of Trans-Himalaya. Int. J. Bot. 6:151-156.
Şimşek. Ö., Biçen. B., Dönmez. D. and Kaçar. YA. 2017. Effects of different media on micropropagation and rooting of Myrtle (Myrtus communis L.) in in vitro conditions. Int J Environ Agric Res 3.
Singh. S. and Kumar. V. 2021. Biology, genetic improvement and agronomy of Bunium persicum (Boiss.) Fedtsch.: A comprehensive review. J Appl Res Med Aromat Plants 100304. https://doi.org/10.1016/j.jarmap.2021.100304.
SOFI. PA., Zeerak. NA. and Singh. P. 2009. Kala zeera (Bunium persicum Bioss.): a Kashmirian high value crop. Turk J Biol. 33:249-258.
Tian. Q., Chen. F., Liu. J., Zhang. F. and Mi. G. 2008. Inhibition of maize root growth by high nitrate supply is correlated with reduced IAA levels in roots. J Plant Physiol. 165:942-951. https://doi.org/10.1016/j.jplph.2007.02.011
Valizadeh. M. and Kazemi. TS. 2009. Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch. J Agric Sci Technol. 11:481-486.
Vandelook. F., Bolle. N. and Van Assche. JA. 2007. Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-Atlantic genus. Ann Bot. 100:233-239. https://doi.org/10.1093/aob/mcm090.
Vlizadeh. M., Safarnejad. A., Nematzadeh. G. and Kazemitabar. S. 2006. Regeneration of plantlets from embryo explants of Bunium persicum (Boiss.) B. Fedtsch. Indian J Agric Sci. 1: 93-96
Yoon. KS. And Leung. DW. 2004. Relative importance of maltose and sucrose supplied during a 2-step potato microtuberization process. Acta Physiol Plant. 26:47-52. https://doi.org/10.1007/s11738-004-0043