QTL mapping for heat tolerance related traits using backcross inbred lines in wheat (Triticum aestivum L.)

Abstract

Heat stress is one of the most limiting factors for the production of wheat. Global warming and consequent changes in climate adversely affect wheat plant growth and yield. To elucidate genetic basis and map heat tolerance traits, a set of 134 backcross inbred lines (BILs) derived from the cross between WH730/*2 HD2733 was used. The population was evaluated under late sown (LS) and very late sown (VLS) conditions, by exposing to heat stress during rabi season. Positive association of normalized difference vegetation index (NDVI), thousand grain weight (TGW), grain weight per spike (GWS), biomass and grain yield (GY) under both production conditions was observed. However, canopy temperature (CT) and days to heading (DH) showed negative correlation with GY under heat stress. A total of 9 Quantitative trait loci (QTL) were discovered on 7 chromosomes, which includes 4 QTLs in LS and 5 QTLs under VLS condition. Combining the results of these QTLs revealed a major stable QTL for DH (qDH_iari_5A) on chromosome 5A with 23% and 26% explaining phenotypic variance under both sowing conditions. QTL for NDVI was detected on chromosome 1B while QTL for SL and GY on chromosome 2A. The identified QTLs in the genomic regions could be targeted for genetic improvement and marker assisted selection for heat tolerance in wheat.