The Efficacy of Molecular Markers-linked to Ryadg Gene in Potato (Solanum tuberosum L.) Genotypes

Abstract

Potato virus Y (PVY) is one of the most important viruses in potato production. The use of resistant varieties is the most effective method to control this virus and, in potato breeding programs, Ryadg is one of the most widely used resistance genes against PVY. Although several molecular markers linked to Ryadg have been developed, however, they have not been compared with each other. In this study, five molecular markers RYSC3, ADG2 Bbvl, ADG1, ADG2 and M45, developed earlier, have been evaluated in potato genotypes inoculated with PVY. In addition, markers GP-122718, GP-122564 and YES 3-3B for Rysto and RY186 for Rychc were used to support the results of the markers related to the Ryadg gene. All markers yielded DNA fragments when potato genotypes were tested, but only the M45 marker was compatible with results of the bioassay. The results of present study showed that the predictive nature of the molecular markers can change according to the genetic background of the potato cultivar/line and may be incorrect with some genotypes.