Molecular characterization of Indian Dolichos bean {Lab/ab purpureus L. var. typicus Prain) accessions using RAPD markers

Abstract

In this study, 29 genotypes of (Lab/ab purpureus) Indian bean augmented from different geographical regions were analyzed for genetic diversity using 39 RAPDs. The binary matrix data revealed 97 .4% polymorphism among genotypes, with 2322 amplification fragments. The PIC values ranged from 0.25 to 0.9 6 with an average of O. 70. Markers OPA05, OPB15, and OPV12 showed high discrimination among all the genotypes. A magnificent range of Jaccard's genetic similarity coefficient was evaluated and UPGMA based dendrogram classified genotypes into 4 major clusters. lC-384066 and NSJ/NAIP/ 192 were found to be unique and highly diversified. The same grouping patterns were also observed in the modelbased STRUCTURE analysis (K=2). The study showed the degree and distribution of genetic diversity in Dolichos bean, which can be utilized to identify the parental lines, to develop mapping populations and breeding.