Molecular cloning and in-silico characterization of gene encoding eukaryotic translation initiation factor (eIF4E) from Capsicum chinense Jacq.
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Abstract
Capsicum chinense Jaqc., is well known for its high capsaicin content and medicinal properties. The productivity of this crop is extensively hindered by different viruses, among which potato virus Y (PVY) is one of the most devastating viruses worldwide. The interaction between eukaryotic translation initiation factor 4E (eIF4E) and potyvirus VPg (viral protein genome-linked) correlates with potyvirus infectivity. Cloning and characterization of the eIF4E gene related to Capsicum viral disease resistance is critical for understanding plant-pathogen interactions, especially with potato virus Y infection. The present study aims to isolate, clone, and characterize the full-length gene encoding eIF4E from C. chinense Jaqc. The full-length eIF4E gene sequence obtained was 692 bp (GenBank Accession No.: MN661348) with an ORF of 687 nucleotides and 228 amino acid residues and blast homology analysis of the CceIF4E sequence showed 99% sequence similarity with C. annum and C. baccatum. A conserved domain was identified from a CDD search. Secondary structure prediction showed 11 alpha helices and seven beta strands. Phylogenetic analysis showed a higher identity to C. annum, indicating the molecular evolution of the CceIF4E gene. The presented work will pave the way for reverse engineering for the development of viral resistance in Bhut Jolokia.
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