Heterogenous inbred families-derived Near Isogenic Lines for growth habit in Dolichos bean [Lablab purpureus (L.) Sweet]

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Published: Dec 26, 2024
DOI: https://doi.org/10.31742/ISGPB.84.4.13
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Mugali Pundalik Kalpana
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Sampangi Ramesh
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Chindi Basavaraj Siddu
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Gonal Basanagouda
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
K. Madhusudan
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Hosakoti Satish
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Narayanaswami Karthik
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Dinesh Sindhu
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Munegowda Kemparaju
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Rotti Kirankumar
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
Basalapura Rangegowda Chandana
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.
J. Venkate Gowda
Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Bangalore 530 065, Karnataka, India.

Abstract

Identification of markers linked to loci controlling economically important traits, including growth habits, helps the selection of genotypes with desired growth habits at the seedling stage itself in crops, with no exception of the dolichos bean. Near isogenic lines (NILs) differing for a target locus are the most appropriate genetic resources for the identification of dependable genomic resources for use in a marker-assisted selection of genes controlling growth habit. In the present study, we found that 30 F2:5 progenies derived from a cross between determinate and indeterminate parents segregated for a few morphological traits, including growth habit. A total of 30 F2:5 progenies were regarded as heterogeneous inbred families (HIFs). Among these 30 F2:5 HIF, seven were segregated only for growth habits. From these seven HIFs, seven pairs of plants differing in growth habit were selected and were regarded as candidate NILs for growth habit. Of these, only six pairs were segregated for growth habit as inferred by polymorphism for alleles at SSR marker (LPD 19) linked to growth habit. Of these, only four pairs of NILs showed monomorphism (>80%) at most of the 94 background SSR markers and hence were regarded as the most putative NILs. These NILs serve as ideal genetic resources to assess the effect of growth habit genes on non-target traits in the dolichos bean.

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How to Cite
Pundalik Kalpana, M., Ramesh, S., Basavaraj Siddu, C., Basanagouda, G. ., Madhusudan, K., Satish, H., Karthik, N., Sindhu, D., Kemparaju, M. ., Kirankumar, R., Chandana, B. R., & Gowda, J. V. (2024). Heterogenous inbred families-derived Near Isogenic Lines for growth habit in Dolichos bean [Lablab purpureus (L.) Sweet]. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 84(04), 630–634. https://doi.org/10.31742/ISGPB.84.4.13
Issue
Vol. 84 No. 04 (2024): Indian Journal of Genetics and Plant Breeding
Section
Research Article
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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