Marker-assisted introgression lines of elite Indian mustard (Brassica juncea) cultivars for resistance against white rust (Albugo candida)

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V.V. Singh
Monika
Priyamedha
Balbeer
M.L. Meena
Pankaj Sharma
R.K. Rai

Abstract

White rust caused by Albugo candida is a major biotic constraint responsible for significant yield loss in mustard production across all mustard-growing regions. This experiment was conducted with the aim of developing WRR lines of Indian mustard using the MABB approach. The elite mustard cultivars of Brassica juncea viz., DRMR150-35, DRMRIJ-31 and NRCDR-02 were used as the recurrent parent, whereas BioYSR, BEC-144 and Donskaja were taken as donors. A parental polymorphism survey using 315 microsatellites (SSR) was done among recurrent and donor parents. To confirm introgression of white rust resistance gene foreground selection was performed using Arabidopsis-derived IP markers that are linked to the white rust loci AcB1-A5.1 (At2g36360) for Donskaja and BioYSR and AcB1-A4.1 (At5g41560) for BEC-144coupled with background selection and phenotypic selection for highest recurrent parent genome recovery. Finally, 19 BC3F3 lines were obtained, which led to the identification of 5 highly white rust-resistant lines with RPG recovery percentage of ˃90%. These selected improved lines showed a resistance response with mean %WR severity (100 DAS) below 5% and possess the agro-morphological characters at par to their respective recurrent parent. These lines have good potential for future release and demonstrated MABB as a valuable tool for expediency, enrichment and precision in accelerating the development of new disease-resistant Indian mustard varieties.

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How to Cite
V.V. Singh, Monika, Priyamedha, Balbeer, M.L. Meena, Pankaj Sharma, & R.K. Rai. (2024). Marker-assisted introgression lines of elite Indian mustard (Brassica juncea) cultivars for resistance against white rust (Albugo candida). INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 84(02), 232–241. https://doi.org/10.31742/ISGPB.84.2.11
Section
Research Article