The effect of chemical-induced mutagenesis in indica rice variety IR50404 for blast disease caused by the Magnaporthe grisea in Vietnam

Abstract

Indica rice variety, IR50404 is the most-grown rice in Vietnam’s Mekong Delta. It is susceptible to multiple blast diseases, making it an ideal genotype for identifying pathological trait mutations. Chemically induced mutant lines were derived from IR50404 by treating seeds with ethyl methanesulfonate (EMS) (0.1 and 0.125%), methyl salicylate (MeSA) (0.1 and 0.125 mM), and their combinations. Chlorophyll, proline, and protease enzyme inhibitors were examined for disease tolerance in mutant rice-based plants. Blast resistance (41.6%) and protease inhibition (30.4%) were highest in T5 (0.1% EMS + 0.1 mM MeSA). Proline (3.91 g/mL) and chlorophyll (8.52 g/mL) concentrations were significantly higher after 0.125% EMS treatment than in the untreated control.In addition, a high rate of protease inhibition (30.4%) was improved after treatment with 0.125% EMS + 0.125 mM MeSA. Results showed that blast resistance was improved by 0.1% EMS and MeSA.This also has implications for the breeding of plant varieties other than rice