Comparative assessment of genetic purity of cotton hybrids by GOT and RAPD markers

Abstract

In the present investigation, a comparative study was made between gro-out test (GOT) and molecular markers in assessing genetic purity in the seeds of hybrid cotton. In GOT, the genetic purity was detected based on color of the anthers, while in molecular studies 20 RAPD markers were used for the same purpose. The genetic purity in the seeds of hybrid Vishwanath ranged from 92.9 to 95.1%, while the same through RAPD markers (OPA-07) ranged from 94 to 96%. Results obtained in seeds lot of hybrid DCH-32 and Varalaxmi were similar. The results of GOT and RAPD markers were comparable. RAPD can be an efficient replacement for otherwise tedious and time consuming GOT tests.