Genomic variation studies in durum wheat (Triticum turgidum ssp. durum) using CBDP, SCoT and ISSR markers

Abstract

In the present study three DNA marker systems viz., start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP) and inter-simple sequence repeats (ISSR) markers were used to detect the genetic diversity and relationship among 17 durum wheat genotypes. The ISSR primers generated 130 bands across the genotypes with an average of 8.12 bands per primer, whereas CBDP and SCoT primers created 66 and 99 polymorphic bands with an average of 5.5 and 7.07 bands per primer, respectively. Values of MI and PIC obtained for all three marker systems indicated the high efficiency of these markers to detect the genetic variation in durum wheat. The results revealed that ISSR markers with an average of 83.46% polymorphism was a suitable marker system to detect genetic variation among genetic materials. On the other hand, gene targeted markers (SCoT and CBDP), similar to ISSR marker, were comparatively less efficient techniques to estimate the genetic variation. The present findings revealed that the genetic analyses using gene targeted markers such as CBDP and SCoT would be more useful for crop improvement programs.