Identification of genotype-specific RAPD markers for Asparagus racemosus

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Published: Nov 25, 2013
DOI: https://doi.org/10.5958/j.0975-6906.73.4.072
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Keywords:
Medicinal herb, molecular marker, authentication, conservation

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Akansha Singh
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005
Ved Prakash Rai
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005
B. Sinha
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005

Abstract

Twenty five diverse genotypes of Asparagus racemosus, were analyzed by RAPD markers for identifying genotypespecific markers. Out of 100 RAPD primers screened, only 25 were polymorphic. Among a total of 197 RAPD fragments amplified, 144 bands (73.09 %) were found polymorphic, revealing high intra-species genetic variation. Eight genotype-specific bands specific to seven A. racemosus genotypes were identified. These genotype-specific RAPD markers could potentially be used for genotype identification, their documentation, management, cultivation and conservation.

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How to Cite
Singh, A., Rai, V. P., & Sinha, B. (2013). Identification of genotype-specific RAPD markers for Asparagus racemosus. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 73(04), 462–465. https://doi.org/10.5958/j.0975-6906.73.4.072
Issue
Vol. 73 No. 04 (2013): Indian Journal of Genetics and Plant Breeding
Section
Research Article
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