Use of amino acids and silicon carbide fibers for improving in vitro plant regeneration and Agrobacterium mediated genetic transformation in Sudan grass [Sorghum sudanense (Piper)]

Abstract

Immature caryopses (10-14 DAP) of a genotype (SDSL 92115) of Sudan grass [Sorghum sudanense (Piper)] were aseptically inoculated on to MS agar media supplemented with 2,4-D (2 mg/I), kinetin (0.5 mg/I) and various concentrations (200, 500 or 1000 mg/I) of proline and/or asparagine. The cultures were incubated in dark at 25 ± 2°C. The first sub-culturing was made to the same medium on which callus was induced. For plant regeneration same basal callusing medium (except for 2,4-D and kinetin) with 2 mg/I IAA and 1 mg/I kinetin was used. Seven to eight weeks old callus from each of the explants was cut into small pieces and put on the regeneration media. The cultures were incubated at 25± 2°C in light (16 hrs photoperiod, 2500-3000 lux) under fluorescent lamp.