Pyramiding of leaf rust resistance genes Lr9 and Lr24 through molecular marker assisted selection in wheat (Triticum aestivum L.)

Abstract

Leaf rust (Puccinia triticina Eriks.) occurs throughout the wheat (Triticum aestivum L.) growing regions of the world. Cultivation of resistant varieties is the most effective, eco-friendly and economically viable method of combating the rust diseases. It is important that the genetic base of rust resistance in the cultivated varieties be widened and also develop lines with more than one effective resistance gene. It is, however, difficult to ascertain successful incorporation of two or more genes through conventional techniques of Host Pathogen Interaction. The presence of such gene, in the absence of differential interaction, can be detected through molecular markers. In the ensuing work, molecular marker reported for Lr9, which was already validated in the parents, was utilized and genetic stock with combined resistance of Lr9 + Lr24 was developed through combination of Host Pathogen Interaction and molecular marker.