Amplified fragment length polymorphism (AFLP) based diversity in advanced breeding lines of cotton (Gossypium hirsutum L.)

Abstract

Amplified fragment length polymorphism (AFLP) analysis was carried out in 29 advanced breeding lines of cotton (G. hirsutum L.) for diversity analysis. Total genomic DNA isolated following CTAB method was purified and subsequently used for AFLP analysis employing fluorescent dye labeling and detection technology. Using 308 amplicons generated by three AFLP primer combinations, Jaccard's similarity estimates between all possible pairs of 29 lines were calculated. High level of diversity was observed in the studied material. Cultivars Pusa 8-6 and RS 810 had the maximum dissimilarity between them, whereas lines RAH 30 and NOH 1001 were naving the highest similarity. Cluster analysis revealed lines AKH 081, NOH 1010, RS 810, RST 13 and Pusa 8-6 to be the most distinct ones. Advanced breeding lines from different sources were found to be interspersed and no source-wise clustering was evident.