ISOZYMIC VARIATION IN RELATION TO YELLOW MOSAIC DISEASE IN SOYBEAN

Abstract

Electrophoretic variations were used to identify yellow mosaic resistant, tolerant and susceptible soybean lines at early stages of the plant growth. Five enzyme systems, i.e., peroxidase (Po), catalase (Cat), polyphenoloxidase (Ppo), esterase (Est) and ribonuclease-I (RNase) were studied. The Po bands numbers 5, 7, 8 and 12 in 6-day-old cotyledons; Cat 1, 2, 3 and 4 in 3, 6 and 12-day-old tissues; Ppo 6, 7, 9 in 3 and 6-day-old tissues; Est 1, 3, 5, 6 and 8 in 3 and 6-day-old seedlings and RNase 1, 2, 3, 4 and 5 in 3, 6 and 12-day-old tissues were the most prominent bands which can be used as biochemical markers for identification of resistant, tolerant and susceptible soybean lines at these specific stages.