EFFECT OF GENOTYP~S AND NUTRIENT MEDIA ON IMMATURE EMBRYO CULTURE OF WHEAT

Abstract

Immature embryo of 14 genotypes of Triticum aestivum and 4 genotypes of T. durum were cultured on four modified MS mediumsupplemented with 30 g.F1sucrose, 8 g.1-1 agar and different level of growth hormones, viz. 2 mgr1 2,4-D; 5 mg.F1 2,4-D; 2 mgr1 2, 4- D + 100 ml.F1 coconut milk; and 2 mg.F 1 2,4-D + 0.5 mg.F 1 kinetin. Highly significant differences in the response of genotypes, culture media and genotype X medium interactions were observed for callus initiation from immature embryos, formation of embryogenic calli and plantlet regeneration.