EFFECT OF 2,4-DICHLORO-PHENOXYACETIC ACID ON BARLEY ANTHER CULTURE IN LIQUID AND AGAR MEDIA

Abstract

Anthers from two cultivars of barley (Hordeum vulgare 1.), Chemigovski 5and Oneprovski 435, were cultured on N6 liquid and semisolid agar media with three different levels of 2,4-0 (2.0, 4.0 and 6.0 mgllitre) and 0.5 mgllitre kinetin. Significant results were obtained for androgenic calli and embryoid production with increased 2,4-0 level, especially in liquid medium preconditioned with isolated earheads. As a result, numberof plantlets was also high although not much difference was observed among media supplemented with three levels of 2,4-0 for the regeneration of green plantlets. Increased 2,4-0 produced more androgenic calli than embryoids. The plantlets regenerated were mostly dihaploids (2n = 2x = 14) and ranged from 65.7% to 84.6% for both the cultivars.