Development and characterization of STMS molecular markers and genetic diversity analysis of mungbean [Vigna radiata (L.) Wilczek]

Abstract

Mung bean is an important crop in Asia because of its high protein content and other economic uses. However, because of the unavailability of polymorphic DNA markers, genomic research of mung bean is lacking. In this study, we developed and characterised simple sequence repeat (SSR) molecular markers by screening SSR-enriched partial genomic libraries with SSR probes and used them to analyse the genetic diversity of mung bean. Thus, we isolated, cloned, sequenced a genomic library that contained microsatellite loci from the mung bean variety ‘MCV-1’. The polymorphisms of microsatellite loci were evaluated using the unweighted pair group method of arithmetic means, and MDS cluster analysis showed genetic relationships in a panel of 96 mung bean core collection genotypes. Genetic diversity analysis results showed contrasted levels of variability within cultivated and wild accessions. A total of 98 alleles were detected using 19 polymorphic markers, with an average of 4.9 alleles per locus, whereas observed heterozygosity ranged from 0.1 to 0.5, with a mean of 0.42 per locus. The number of alleles and the high level of polymorphism make these new markers useful for gene tagging, diversity analyses and marker assisted selection in mung bean.