Assessment of genetic diversity in cultivated groundnut (Arachis hypogaea L.) with differential responses to rust and late leaf spot using ISSR markers
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Abstract
Inter simple sequence repeat (ISSR) markers were used to analyze genetic diversity among twenty cultivated groundnut (Arachis hypogaea L.) genotypes. Out of 154 amplicons produced by 21 ISSR primers, 115 were found polymorphic (74.67%). The 3’-anchored primers based on poly ‘GA’ and poly ‘AG’ motifs produced higher proportion of polymorphism of 74.85% and 77.27%, respectively. The primer UBC 810 identified 18 genotypes out of 20 studied as it had the highest resolving power among the 21 primers. The dendrogram obtained from Ward method of cluster analysis revealed four subclusters, of which subcluster A and subcluster D contained resistant and susceptible genotypes, respectively. Based on Kruskal- Wallis one-way ANOVA, UBC 810540 was found associated with both rust and late leaf spot (LLS) resistance and UBC 810500 with LLS resistance.
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How to Cite
Mondal, S., Sutar, S. R., & Badigannavar, A. M. (2009). Assessment of genetic diversity in cultivated groundnut (Arachis hypogaea L.) with differential responses to rust and late leaf spot using ISSR markers. INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 69(03), 219–224. https://doi.org/.
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Research Article
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