Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.)
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Abstract
Efficient plant regeneration via somatic embryogenesis has been developed in chickpea from embryonic axes on MS medium supplemented wth 1.25 mgll 2, 4-0 and 0.25 mgn, kinetin induced callusing after 7 days of culture. On the same medium, 7 days old callus masses started differentiating with high efficiency of globular structures during dark incubation. 1/2 MS salts + B5 vitamins + 40 gil, sucrose was found suitable for embryo maturation. The well formed embryos germinated into plantlets on MS salts + B5 vitamins + 1.25 mgll IBA + 2.0 mgll BAP. Normal plants were transferred to soil.
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How to Cite
Chauhan, R., & Singh, N. P. (2002). Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.). INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 62(04), 319–321. https://doi.org/.
Section
Research Article
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